@article {717, title = {In vitro antiproliferative activity of Annona reticulata roots on human cancer cell lines}, journal = {Pharmacognosy Research}, volume = {3}, year = {2011}, month = {April 2011}, pages = {09-12}, type = {Original Article}, chapter = {09}, abstract = {

Background:\ The phytochemical and pharmacological activities of\ Annona reticulata\ components suggest a wide range of clinical application in lieu of cancer chemotherapy.\ Materials and Methods:\ Ethanol and aqueous extracts of roots of\ Annona reticulata\ Linn were studied for their\ in vitro\ antiproliferative activity on\ A-549\ (human lung carcinoma),\ K-562\ (human chronic myelogenous leukemia bone marrow), HeLa\ (human cervix) and\ MDA-MB\ (human adenocarcinoma mammary gland) cancer cell lines by MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] colorimetric assay.\ Results:\ The ethanol extract exhibited a prominent inhibitory effect against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines at a concentration range between 10 and 40 μg/ml, whereas the aqueous extract showed a lower activity at the same concentration. Simultaneously, the effect of the ethanol extract toward the inhibition of\ Vero\ cell line proliferation was lower in comparison with the cancer cell lines.\ Conclusion:\ The significant antiproliferative activity of the ethanol extract of\ Annona reticulata\ roots against\ A-549, K-562, HeLa\ and\ MDA-MB\ human cancer cell lines may be attributed toward the collective presence of acetogenins, alkaloids and lower inhibitory effect on\ Vero\ cell line, which suggests\ Annona reticulata\ be used as a chemopreventive agent in cancer therapy.

}, keywords = {A-549, Annona reticulata, Antiproliferative activity, HeLaand MDA-MBhuman cancer cell lines, K-562, MTT assay}, doi = {10.4103/0974-8490.79109}, author = {HM Suresh and B Shivakumar and K Hemalatha and SS Heroor and DS Hugar and K.R.S Sambasiva Rao} }