@article {515, title = {Evaluation of In vitro Antiviral Activity of Datura metel Linn. Against Rabies Virus}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {August 2016}, pages = {265-269}, type = {Original Article}, chapter = {265}, abstract = {

Objective: The soxhlet and cold extracts of Datura metel Linn. were evaluated for in vitro antirabies activity. Materials and Methods: Soxhlet and cold extraction method were used to extract Datura (fruit and seed) extracts. In vitro cytotoxicity assay was performed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. Based on the CC50 range, the in vitro antirabies activity of the extracts was screened by rapid fluorescent focus inhibition test and molecular method. Results: The Datura (fruit and seed) extracts were not cytotoxic below 5 mg/ml (CC50). Titer of 10-4 rabies virus challenge virus standard (RV CVS) (1 50\% tissue culture infective dose [1 TCID50]) was obtained by RFFT method and the challenge dose of 10 TCID50 was used for antirabies assay. Datura fruit and seed (soxhlet and cold) extracts showed 50\% inhibition of RV CVS at 2.5 mg/ml and 1.25 mg/ml (inhibitory concentration 50\% [IC50]), respectively. The tested extracts showed selectivity index (CC50/IC50) ranging from 2 to 4. The viral RNA was extracted and real-time reverse transcription-polymerase chain reaction was performed which also revealed a 2-fold reduction of viral load at 1.25 mg/ml of the Datura seed (soxhlet methanolic and cold aqueous) extracts. Conclusion: To the best of our knowledge, this is the first study of in vitro antiviral activity of D. metel Linn. against rabies virus. Datura seed extracts have a potential in vitro antirabies activity and, in future, can be further screened for in vivo activity against rabies virus in murine model.

}, keywords = {Antirabies, Antiviral assay, cytotoxicity, Datura, Rapid fluorescent focus inhibition test}, doi = {10.4103/0974-8490.188874}, author = {Soumen Roy and Sandeepan Mukherjee and Sandip Pawar and Abhay Chowdhary} }