@article {484, title = {Characterization of Anticancer Principles of Celosia argentea (Amaranthaceae)}, journal = {Pharmacognosy Research}, volume = {8}, year = {2016}, month = {February 2016}, pages = {97-104}, type = {Original Article}, chapter = {97}, abstract = {

Background: An Indian origin, Celosia argentea is a weed growing during rainy season traditionally claimed for treating several ailments. Early researches on C. argentea were focused on the anti-cancer screening of seeds, with few reports on aerial parts. Objective: To isolate and characterize bioactive compounds of aerial parts of C. argentea and evaluate their anticancer potential. Materials and Methods: The methanolic aerial part extract was fractionated on column chromatography using chloroform: methanol mixture. The fractions; 80:20 and 95:5 were purified on MCI-HP20 HPLC column. Chromatographically pure compounds were pooled, concentrated and characterized spectroscopically. The compounds were further screened for antioxidant and cytotoxic potential. Results: Isolated compounds were confirmed as: (1) Luteolin-7-O-glucoside and (2) phenolic, 1-(4-hydroxy- 2-methoxybenzofuran-5-yl)-3-phenylpropane-1,3-dione. Both exhibited significant antioxidant potential with IC50 values of 20.80 and 21.30 μg/ml for 2,2-diphenyl-1-picrylhydrazyl assay (***P \< 0.001) and significant Trolox equivalent antioxidant capacity (TEAC) values for 2,2{\textquoteright}-azino-bis(3- ethylbenzthiazoline-6-sulphonic acid) (*P \< 0.05) and ferric reducing antioxidant potential assay (****P \< 0.0001). In 3(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazoliumbromide assay, Compound 1 and 2 showed potent cytotoxicity against SiHa, HCT, MCF-7 cancer cell lines at 20 μg/ml (****P \< 0.0001) and 18 μg/ml (**P \< 0.01), respectively, without affecting the normal Vero cells. Both compounds enabled maximum reduction in cell viability at 50 μg/ml against HT-29 (***P \< 0.001) and MCF-7 cell lines (**P \< 0.01) in try pan blue viability assay. Apoptosis occurred at concentrations of 47.33 {\textpm} 0.8 μg/ml and 56.28 {\textpm} 1.2 μg/ml for Compound 1 and 35.15 {\textpm} 0.4 μg/ml and 28.05 {\textpm} 0.3 μg/ml for Compound 2 for HT-29 and MCF-7 respectively. Conclusion: A novel anticancer phenolic compound; (1-(4-hydroxy-2-methoxybenzofuran-5-yl)-3-phenylpropane- 1,3-dione), isolated from aerial parts of C. argentea was a valuable finding of the research.

}, keywords = {2, 2-diphenyl-1-picrylhydrazylDPPH, 3(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyl-tetrazoliumbromideMTT, anti-oxidant, anticancer, Celosia argentea}, doi = {10.4103/0974-8490.172659}, author = {Rukhsana A. Rub and Manohar J. Pati and Areej A. Siddiqui and Alpana S. Moghe and Nasreen N. Shaikh} }