@article {395, title = {Chemical Constituents and Bioactivities of Glinus Oppositifolius}, journal = {Pharmacognosy Research}, volume = {7}, year = {2015}, month = {June 2015}, pages = {138-147}, type = {Original Article }, chapter = {138}, abstract = {

Objectives: To isolate the secondary metabolites from the dichloromethane (DCM) extracts of Glinus oppositifolius; to test for the cytotoxicity of a new triterpene, oppositifolone (1); and to test for the hypoglycemic, analgesic, and antimicrobial potentials of 1, DCM and aqueous leaf extracts of G. oppositifolius. Methods: The compounds were isolated by silica gel chromatography and identified by nuclear magnetic resonance spectroscopy. The cytotoxicity potential of 1 was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Triterpene 1, DCM, and aqueous leaf extracts were tested for hypoglycemic potential using the oral glucose tolerance test; analgesic potential using the tail-flick assay, and antimicrobial potential using the disc diffusion method. Results: The DCM extracts of G. oppositifolius afforded 1, squalene, spinasterol, oleanolic acid, phytol, and lutein from the leaves; squalene and spergulagenin A from the stems; and spinasterol from the roots. Triterpene 1 was cytotoxic against human colon carcinoma 116 with an IC50 value of 28.7 but did not exhibit cytotoxicity against A549. The aqueous leaf extract at 200 mg/kg body weight (BW) exhibited hypoglycemic activity with a pronounced \% blood glucose reduction of 70.76\% {\textpm}17.4\% within 0.5 h after introduction. The DCM leaf extract showed a lower \% blood glucose reduction of 18.52\% {\textpm}13.5\% at 200 mg/kg BW within 1.5 h after introduction, while 1 did not exhibit hypoglycemic activity. The samples did not exhibit analgesic property and were inactive against multiple drug resistant bacterial pathogens. Conclusion: The compounds responsible for the hypoglycemic activity of G. oppositifolius which are fast acting (0.5 h) are found in the aqueous leaf extract.

}, keywords = {3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Analgesic, Antimicrobial, Cytotoxic, Glinus oppositifolius Linn, Hypoglycemic, Molluginaceae, Oppositifolone}, doi = {10.4103/0974-8490.150520}, author = {Consolacion Y. Ragasa and Esperanza C. Cabrera and Oscar B. Torres and Adiel Inah Buluran and Dinah L. Espineli and Dennis D. Raga and Chien-Chang Shen} }