@article {300, title = {Determination and Quantification of p-Coumaric Acid in Pineapples (Ananas comosus) Extracts using Gradient Mode RP-HPLC}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {February,2019}, pages = {67-71}, type = {Original Article}, chapter = {67}, abstract = {

Background: Pineapple (Ananas comosus) is a good source of phenolic compounds such as p-coumaric acid.\ P-Coumaric acid is a hydroxycinnamic acid known to possess antioxidant, antimicrobial, antimutagenic, and immunoregulatory activities. Objective: The present study aims to determine and quantify the p-coumaric acid in the juice extract and methanol extract of unripe and ripe pineapples by gradient mode reverse-phase high-performance liquid chromatography (RP-HPLC). Materials and Methods: The analysis was carried out using 0.5\% phosphoric acid and 100\% acetonitrile (can) as mobile phase A and B at gradient elution conditions of 0{\textendash}30 min from 5\% B to 80\% B, 30{\textendash}33 min at 80\% B, 33{\textendash}35 min from 80\% B to 5\% B, and 35{\textendash}40 min at 5\% B. The chromatography separation was performed using Gemini C18 column at flow rate of 0.8 mL/min and detection was made at 280 nm. Results: P-Coumaric acid was determined at retention time of 16.16 min. Standard curves were linear at R 2 = 0.9973 with low limit of detection and limit of quantification being 0.0208 and 0.0694, respectively. P-Coumaric acid showed the highest concentration in juice extract of ripe pineapple, 11.76 μg/mL compared to the methanol extract, 0.03 μg/mL, respectively. P-Coumaric acid concentration in juice extract of unripe pineapple was 0.41 μg/mL. Conclusion: The gradient mode RP-HPLC proved to be efficient for determination and quantification of p-coumaric acid in pineapples extracts.

}, keywords = {Ananas comosus, Gradient mode, Pineapple, p-coumaric acid, Reverse-phase high-performance liquid chromatography.}, doi = {10.4103/pr.pr_154_18}, author = {Wan Mazlina Md Saad and Rasdin Ridwan and Nur Syamimi Md Lasim and Nur Liyana Mohd Rapi and Fatimah Salim} }