@article {188, title = {Effect of Geraniol and Clarithromycin Combination against Gastric Ulcers Induced by Acetic Acid and Helicobacter pylori in Rats}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {November,2019}, pages = {356-362}, type = {Original Article}, chapter = {356}, abstract = {

Background: Previous work showed geraniol to have anti-Helicobacter pylori activity in vivo and in vitro. Further, it has augmented the anti-H. pylori activity of clarithromycin in vitro. Objective: Geraniol and clarithromycin combination was investigated for anti-ulcer and anti-H. pylori activity in rats. Materials and Methods: Ulcers were induced by injecting acetic acid into the subserosal layer of the stomach followed by intragastric inoculation of H. pylori for 7 days. Geraniol (30 mg/kg) and a drug combination (geraniol, 30 mg/kg and clarithromycin, 25 mg/kg) were administered twice daily for 14 days, and parameters were measured at the end of treatment. Results: Geraniol alone or in combination with clarithromycin significantly (P \< 0.05) lowered the ulcer index and improved the curative ratio. Histopathological examination showed a significant reduction (P \< 0.05) in the ulcer score in the geraniol and geraniol{\textendash}clarithromycin treated animals compared with the ulcer controls. Both the treatment groups showed significant (P \< 0.05) increase in the glutathione level and reduction in the malondialdehyde, interleukin 6, and tumor necrosis factor-α level in the gastric mucosa compared to control group. Both produced a 50\% reduction in the rapid urease test, which is further confirmed using modified Giemsa staining. Conclusion: Geraniol, alone or in combination with clarithromycin, showed a significant ulcer healing effect which was associated with anti-H. pylori, anti-inflammatory, and antioxidant effects in the gastric mucosa. However, the effects of the geraniol and clarithromycin combination were not significantly different from the effects of geraniol alone in this model.

}, keywords = {Antioxidant, Antiulcer, Anti-inflammatory, Clarithromycin, Geraniol, Helicobacter pylori.}, doi = {10.4103/pr.pr_21_19}, author = {Subrat Kumar Bhattamisra and Lee Peng Hooi and Lai Pey Shyan and Lee Boon Chieh and Mayuren Candasamy and Priyadarshi Soumyaranjan Sahu} }