@article {163, title = {Biological Screening of Tri-Jannarose as a Recipe from Thai Traditional Medicine}, journal = {Pharmacognosy Research}, volume = {11}, year = {2019}, month = {April 2019}, pages = {110-114}, type = {Original Article}, chapter = {110}, abstract = {

Context: Tri-Jannarose (TJ) is a Thai traditional medicine recipe, the ingredients of which are betel palm seed (Areca catechu L.), Siamese neem tree root (Azadirachta indica A. Juss.), and heart-leaved moonseed vines (Tinospora cordifolia [Thunb.] Miers). The equal mixture of three plants indicated to treatment of antipyretic, diuretic, expectorate, nourishment and appetizing. Aims: Phytochemical screening, antioxidant, and α-glucosidase inhibitory activities of TJ using different extractions were evaluated. Materials and Methods: The three plants of recipe were extracted using different solvents such as aqueous extract (ATJ), 50\% ethanolic extract (HETJ), and 95\% ethanolic extract (ETJ). The phytochemical screening was determined by total phenolic compounds and total flavonoid contents. The anti-oxidation were tested using by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and 2,2 -azinobis- (3-ethylbenzothiazoline-6-sulphonate) (ABTS+) assay. The α-glucosidase inhibitory activity was determined for glucose transferase mechanism. Results: Phytochemical screening found that this recipe had both phenolic and flavonoid substances. The ETJ (IC50 = 0.0463 {\textpm} 0.002) was exerted on antioxidation higher than HETJ and ATJ (IC50 = 0.0511 {\textpm} 0.000 and 0.1485 {\textpm} 0.005 mg/mL). Surprisingly, ABTS+ assay, ETJ (IC50 = 0.015 {\textpm} 0.000 mg/mL), and HETJ (IC50 = 0.022 {\textpm} 0.000 mg/mL) showed high effect on free radical scavenging activity than the standard controls, ascorbic acid (IC50 = 0.025 {\textpm} 0.001 mg/mL), and Trolox (IC50 = 0.032 {\textpm} 0.001 mg/mL). The α-glucosidase inhibitory activity found that all of the extract including ATJ (IC50 = 0.0127{\textpm}0.02 mg/mL), ETJ (IC50 = 0.0154{\textpm}0.01 mg/mL) and ETJ (IC50 = 0.0202{\textpm}0.01 mg/mL) were more potent to inhibit α-glucosidase emzyme than acarbose (IC50 =0.745{\textpm}0.026 mg/mL) as a positive control. Conclusion: The pharmaceutical preliminary scarring was confirmed to treatment on Thai traditional medicine. The recipe composed with phenolic compounds and flavonoids contents which chemical substance were more potent anti-oxidation, and bittersweet flavor was stronger to α-glucosidase inhibitory activity.

}, keywords = {Antioxidant, Phytochemical screening, Thai traditional medicine, Tri-Jannarose, α-glucosidase}, doi = {10.4103/pr.pr_178_18}, author = {Ladachart Taepongsorat and Ampa Konsue} }