Synthesis and Pharmacological Evaluation of Novel Formulation of Opiorphin and Lysozyme: An in vitro Study

Background: The antibacterial, anti-inflammatory and antioxidant properties of opiorphin and lysozyme are vital for health maintenance and disease prevention. Opiorphin reduces oxidative stress by neutralizing free radicals and boosting antioxidant enzymes. Lysozyme helps manage inflammation, supporting quicker healing in infections and injuries. These properties protect cellular components from damage, regulate inflammation and promote recovery. Understanding and utilizing these effects can enhance medical treatments and overall health. Materials and Methods: opiorphin is purchased in powder form and lysozyme is obtained from artificial saliva. Anti-inflammatory assay is performed with 0.2 mL of albumin and 3 mL of phosphate buffer in pH of 6.4+combination of opiorphin and lysozyme taken in different concentrations (20, 40, 60 ug/ mL). Likewise, antioxidant assay with 28 mM of sodium phosphate, 0.6M sulphuric acid+, 4M of ammonium molybdate+opiorphin and lysozyme and anti-bacterial assay with freshly prepared nutrient broth of Enterococcus faecalis and Streptococcus mutants. Results: In anti-inflammatory assay, higher concentrations of the substance result in increased absorbance, indicating greater anti-inflammatory activity. Salicylic acid has the highest absorbance, suggesting it is the most effective in this assay. In an antioxidant assay, a minimal increase after increasing concentration is noted, measured by absorbance at 517 nm. Likewise, HC shows larger inhibition zones compared to LC. Conclusion: Lysozyme and opiorphin have numerous beneficial qualities, including antibacterial, anti-inflammatory and antioxidant ones. The findings are quite valuable. Although lysozyme and opiorphin alone have several advantages, when combined, they would improve or synergize the process. To create a medicine, research must be conducted in tandem to determine its pharmacophore and binding energy.