Exploring the Glycosidic Compounds in Ipomoea sagittifolia Stems: A High-Performance Liquid Chromatography Approach

Background: The abstract provides a comprehensive overview of the study on secondary metabolites from Ipomoea sagittifolia, emphasizing the extraction, isolation and characterization processes. Secondary metabolites, including alkaloids, flavonoids, phenols and glycosides, were identified and analyzed using various analytical techniques. Materials and Methods: Ethanol extraction followed by Soxhlet apparatus yielded a crude extract, subsequently purified through column chromatography using a solvent mixture of n-butanol, glacial acetic acid and water. Thin Layer Chromatography (TLC) confirmed the presence of compounds across 108 eluted fractions, further validated by UV-vis spectroscopy revealing absorption peaks characteristic of glycosides at 288 nm. Results: Fourier Transform Infrared (FTIR) spectroscopy highlighted functional groups such as alcohols, phenols, carboxylic acids and ketones, typical of glycoside structures. High-Performance Liquid Chromatography (HPLC) confirmed the presence of cardiac glycosides through retention time analysis, aligning closely with Scillaren A standards. Conclusion: These findings underscore the pharmacological potential of Ipomoea sagittifolia in traditional medicine and pharmaceutical applications, supported by its diverse secondary metabolite profile. Future research could delve deeper into bioactivity assays to elucidate specific therapeutic properties linked to these metabolites, thereby enhancing its relevance in healthcare and biotechnological innovations.