Evaluation of Wound Healing Activity of Polyherbal Gel Containing Azadirachta indica, A. Juss. and Tridax procumbens L. Extracts

Background: Azadirachta indica A. Juss. and Tridax procumbens L. have been utilized for wound management and therapy since ancient times and offer huge potential. Objectives: To formulate polyherbal gel containing Azadirachta indica A. Juss. and Tridax procumbens L. and to evaluate it further in wound management. Materials and Methods: In this study, three polyherbal formulations of Azadirachta indica A. Juss. and Tridax procumbens L. with varied proportions of both plants were formulated and appearance, nature, grittiness, phase separation, pH, viscosity and extrudability of all prepared formulations were compared with control, standard (Betadine 5%) and individual formulations of these plants. Excision wound model was used to study wound healing action of prepared formulation. Results: Formulation with Azadirachta indica A. Juss. and Tridax procumbence L. extracts in equal proportion showed the best results in formulation parameters. The rate of wound contraction, tensile strength, epithelialization rate, and hydroxyproline content of the wound were used to determine the rate of wound healing. According to the results, there was an increase in wound contraction as well as an increase in wound strength for this formulation. Correlations between hydroxyproline expression and healing patterns were also found in histopathology, it was observed that the wounds treated with an equal proportion of plant extract and betadine minimized scar formation and improved fibroblast proliferation, angiogenesis, keratinization, and epithelialization. Conclusion: As per the different physical, histological, and biochemical parameters, hydroalcoholic extract of Azadirachta indica A. Juss. and Tridax procumbens L. in equal proportions showed good wound healing activity.