ArticlesAbstractPharmacognosy Research,2024,16,4,929-934.DOI:10.5530/pres.16.4.106Published:October 2024Type:Original ArticleAuthors:Jyothisree Gangadharan, and Umadevi Sankararajan Author(s) affiliations:Jyothisree Gangadharan, Umadevi Sankararajan* School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, INDIA. Abstract:Background: Ficus religiosa, revered for its medicinal properties in traditional medicine, has drawn scientific interest due to its diverse chemical composition. This study aimed to develop a densitometric High-Performance Thin Layer Chromatography (HPTLC) technique to estimate phenolic compounds present in the hydroalcoholic extract of dried root bark of Ficus religiosa. Materials and Methods: This study utilized HPTLC to identify the constituents within Ficus religiosa root bark. The isolated constituent was evaluated by various analytical methods like phytochemical tests, UV, HPTLC etc. Subsequently, the HPTLC method underwent rigorous validation, confirming its reliability and accuracy in separating and quantifying the constituents within the root bark of Ficus religiosa. Results: Isolation of phytoconstituents was done by column chromatographic technique using toluene: ethyl acetate: methanol (3:3:2). The isolated component was confirmed as a flavonoid moiety by various chemical tests and by analytical evaluation. Also, HPTLC analysis of the isolate revealed the compound was Quercetin a flavonoid moiety or its derivative. Conclusion: These findings underscore the presence of main phytoconstituents in Ficus religiosa root bark which is responsible for pharmacological effects and offers insights into its application in both traditional and contemporary medicinal practices. Keywords:Ficus religiosa, Flavonoids, HPTLC, Quantification, ValidationView:PDF (248.55 KB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images Ficus religiosa plant KeywordsFicus religiosaFlavonoidsHPTLCQuantificationValidation ‹ Evolution and Confirmation of UV Spectrophotometric Approach for Simultaneously Estimating Allicin and Azadirachta indica Extracts in Niosomal Formulation up Evaluating the Efficacy of a Ginger Extract-Infused Mustard Oil Transdermal Patch for Arthritis Management ›