Possible Inhibition of Renal Protein Glycation by Carnosine and Tocopherol in Diabetic Rat

Background: Carnosine is a biological active dipeptide in different tissue exert physiological role as buffer in muscle and cell cycle division. Tocopherol (vitamin E) is the potent antioxidant natural product. This study investigated the role of carnosine supplementation with vitamin E in protection against nephropathy in diabetic rats. Materials and Methods: This study was carried out on 75 rats grouped into five groups; control and the other rats groups (II-V) were received a single dose of streptozocine i.p, at dose of (65 mg/kg/b.w) for diabetogenesis. GPI were considered as diabetic untreated. Rats in groups 3-6 were treated i.p daily with carnosine (1mg/kg b.w), vitamin E (50,00 IU/kg b.w) or combined (10 mg/kg b.w) respectively for 12 weeks. Fasting serum was subjected for assay of glucose, HbA_1c, AGEs, MDA, SOD, total antioxidant activity and inflammatory markers (TNF-α and IL-6). Results: Data obtained showed that, diabetic rats treated with carnosine and Vitamin E showed improvement in glucose, glycated hemoglobin, antioxidant enzymes, decreased inflammatory mediators and AGEs (p <0.001) versus untreated diabetic. In addition, combined treatment is better than individual treatment (p <0.05). Conclusion: Promising positive results were spot a new strategy of carnosine and Vitamin E rich food supplements for protection against diabetic complications. It can be used as complementary and alternative therapy for nephropathy, neuropathy and cardiomyopathy.