Antioxidant and Anti-inflammatory Activity of Mikania glomerata and Mikania laevigata Extracts

Alexandre Augusto Borghi; Elaine Minatel; Daniela Sayuri Mizobuti; Caroline Caramano de Lourenço; Fabio Fernandes de Araújo; Glaucia Maria Pastore; Peter Hewitson; Svetlana Ignatova; Alexandra CHF Sawaya

Antioxidant and Anti-inflammatory Activity of Mikania glomerata and Mikania laevigata Extracts

Articles

Abstract

Pharmacognosy Research,2023,15,1,128-137.

DOI:10.5530/097484900264

Published:December 2022

Type:Original Article

Authors:

Author(s) affiliations:

Alexandre Augusto Borghi¹, Elaine Minatel², Daniela Sayuri Mizobuti², Caroline Caramano de Lourenço², Fabio Fernandes de Araújo⁵, Glaucia Maria Pastore³, Peter Hewitson⁴, Svetlana Ignatova⁴, Alexandra CHF Sawaya^5,*

¹Department of Plant Biology, Institute of Biology, University of Campinas, Campinas, SP, BRAZIL.

²Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, BRAZIL.

³Department of Food Science, Faculty of Food Engineering, University of Campinas, Campinas, SP, BRAZIL.

⁴Department of Chemical Engineering, CEDPS, Brunel University, London, UK.

⁵Faculty of Pharmaceutical Science, University of Campinas, Campinas, SP, BRAZIL.

Abstract:

Background: Mikania glomerata and Mikania laevigata (guaco) extracts are popularly used for the treatment of asthma and cough as well as for their anti-inflammatory activity, indistinctly, despite their different chemical composition. Both species may present these activities however, the specific components and the cellular mechanisms are not fully identified. Objectives: To determine the activity of fractions obtained by countercurrent chromatography pooled based on their TLC and UHPLC-MS chromatographic profiles. Materials and Methods: Fractions with antioxidant activity in DPPH and ORAC tests were assayed in dystrophic primary muscle cell cultures from mdx mice, the experimental model of Duchenne muscular dystrophy (DMD), to evaluate their cellular anti-inflammatory and antioxidant activity. The inflammatory process was evaluated by determining the TNF-α, NF- κB and IL-1β content by immunoblotting; content of 4-hydroxynonenal, superoxide dismutase (SOD); catalase, glutathione peroxidase (GPx); glutathione reductase (GR) and glutathione (GSH) were determined to evaluate their antioxidant activity. Results: The crude M. glomerata and M. laevigata extracts, as well as 3 selected fractions presented antioxidant capacity in the ORAC assay and only Mlet Fr13 did not present activity by DPPH. Immunoblotting revealed no significant differences between the experimental groups, so no cellular anti-inflammatory effect was observed, however, reduced levels of anti-oxidant defence system components were observed for all fractions. Conclusion: Both species contain compounds that effectively reduced anti-oxidant defense system components, but none of these fractions significantly reduced inflammatory markers, suggesting that the reported anti-inflammatory activity of these species may be mediated by oxidative stress reduction.