ArticlesAbstractPharmacognosy Research,2022,14,2,115-120.DOI:10.5530/pres.14.2.16Published:April 2022Type:Original ArticleAuthors:Benjaporn Buranrat Author(s) affiliations:Benjaporn Buranrat Faculty of Medicine Program, Mahasarakham University, Muang District, Maha Sarakham, THAILAND. Abstract:Background: Benjakul is a Thai traditional formulation and has been reported for cancer treatment. Objectives: In this study aimed to examine the cytotoxic effects of Benjakul extract on suppression of breast cancer cells proliferation and migration. Materials and Methods: Cytotoxic effects were determined by sulforhodamine B, colony formation, ROS formation, caspase 3 activity, and mitochondrial function. Migration assay was studied by wound healing and gelatin zymography. Results: Piperine level was showed the highest concentration in the Benjakul extract. Further, Benjakul extract exhibited cytotoxicity against breast cancer cells with low IC50 equally effects in two cancer cells and further it suppressed the colony formation. The mechanism of Benjakul to induce cells death through activating ROS formation, stimulating caspase 3 activity, and repressing mitochondrial function. Moreover, the suppression of migration by Benjakul was acted on reduction of MMP-9 expression level in culture medium. Conclusion: Our findings demonstrated that Benjakul extract can suppress human cancer cells proliferation along with induction of apoptosis and attenuation of migration by low concentration. Key words: Benjakul, Breast cancer cells, Growth, Apoptosis, Migration. View:PDF (304.23 KB) PDF Thumbnails Document Outline Search Document Find Toggle Sidebar Previous Next Page: Fullscreen Print Download Current View Zoom Out Zoom In Automatic Zoom Actual Size Fit Page Full Width 50% 75% 100% 125% 150% 200% More Information Less Information Close Click here to download the PDF file. Images High-performance liquid chromatography chromatogram of active compounds in Benjakul extracts, (A) Standard (B) Benjakul extract. ‹ Traditional and Herbal Medicines: Opportunities and Challenges up In vitro Study of Cnidoscolus aconitifolius Leaf Extracts on Foam Cells and their Antioxidant ›