Short‑term Effects of Date Palm Extract (Phoenix dactylifera) on Ischemia/Reperfusion Injury Induced by Testicular Torsion/ Detorsion in Rats

Background: Antioxidants are potent scavengers of free radicals and have beneficial effects on human health. Objective: The aim of this study was to investigate the potential protective antioxidant activity of the edible portion of date fruit extract in an experimental testicular torsion/detorsion (T/D) model in rats. Materials and Methods: To investigate the potential protective effects of date palm (DP), 30 male Spraque‑Dawley rats were divided into three groups: sham‑operated, T/D, and T/D + DP‑treated (500 mg/kg, PO) groups. Testicular ischemia was induced via keeping the left testis under 720° clockwise torsion for 2 h (h), afterward, detorsion was performed. All rats were sacrificed 4 h after detorsion. Serum malondialdehyde (MDA) concentration, total oxidative status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and histopathological damage score were evaluated. Results: Serum MDA, TOS, and OSI levels rose significantly in the T/D group. These values were lower in the T/D + DP group. TAS values decreased significantly in T/D group and rose in T/D + DP group. Severe injury was seen in the twisted testes of T/D group. In contrast, ipsilateral‑twisted testicular tissue in the DP‑treated group showed moderate‑to‑mild changes. Contralateral testicular tissue in the T/D group had a mild‑to‑moderate tissue injury; meanwhile, treated group revealed normal‑to‑mild changes. Spermatogenesis was significantly improved in DP‑treated group when compared with the T/D group. Conclusion: The findings suggest a possible protective effect of DP against testicular oxidative damage induced by T/D; however, more detailed studies are warranted.