Hepatoprotective and Cytotoxic Activities of Abietic Acid from Isodon wightii (Bentham) H. Hara

Madhusudhanan Gogul Ramnath; Ramaraj Thirugnanasampandan; Sadasivam Mathusalini; Palathurai Subramanium Mohan

Hepatoprotective and Cytotoxic Activities of Abietic Acid from Isodon wightii (Bentham) H. Hara

Articles

Abstract

Pharmacognosy Research,2016,8,3,206-208.

DOI:10.4103/0974-8490.182920

Published:May 2016

Type:Short Communication

Authors:

Author(s) affiliations:

Madhusudhanan Gogul Ramnath¹, Ramaraj Thirugnanasampandan¹, Sadasivam Mathusalini², Palathurai Subramanium Mohan²

¹Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore, Tamil Nadu, INDIA.

²Department of Chemistry, Bharathiar University, Coimbatore, Tamil Nadu, INDIA.

Abstract:

Isodon (Lamiaceae) is a known source of bioactive terpenoids. Diterpenoids isolated from Isodon wightii (Bentham) H. Hara showed antibacterial, antiacetylcholinesterase, antioxidant, anticancer, and anticarcinogenic activities, etc., Hepatoprotective activity of ABA against lipopolysaccharide (LPS) induced liver injury in BALB/c mice was studied. Cytotoxic activity of ABA was tested against cervical cancer cells (HeLa) using MTT assay followed by propidium iodide (PI) staining to identify apoptosis. Histopathological analysis revealed that 1.5 μg/mL LPS induced liver damage was attenuated by ABA in a dose dependent manner. ABA showed cytotoxicity with IC50 value of 176.28 ± 0.02 μg/mL and PI staining of treated cells showed apoptosis. This study proves that ABA would be a promising natural compound for herbal drug preparation.

Keywords:Abietic acid, Apoptosis, BALB/c mice, HeLa, lipopolysaccharide