Isolation and Identification of Bacterial Endophytes from Pharmaceutical Agarwood-producing Aquilaria Species

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Abstract
Pharmacognosy Research,2013,5,2,134-137.
Published:April 2013
Type:Short Communication
Authors:
Author(s) affiliations:

Subhash J Bhore1, Jagadesan Preveena1, Kodi I Kandasamy2


1Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Bedong Semeling Road, Semeling 08100, Kedah, Malaysia
2Tissue Culture Laboratory, Forest Research Institute Malaysia, 52109 Kepong, Selangor; Malaysian Biotechnology Corporation, Level 23, Menara Atlan, 161B, Jalan Ampany, 50450 Kuala Lumpur, Malaysia


 

Abstract:

Background: Resins and gums are used in traditional medicine and do have potential applications in pharmacy and medicine. Agarwood is the fragrant resinous wood, which is an important commodity from Aquilaria species and has been used as a sedative, analgesic, and digestive in traditional medicine. Endophytic bacteria are potentially important in producing pharmaceutical compounds found in the plants. Hence, it was important to understand which types of endophytic bacteria are associated with pharmaceutical agarwood-producing Aquilaria species. Objective: This study was undertaken to isolate and identify endophytic bacteria associated with agarwood-producing seven (7) Aquilaria species from Malaysia. Materials and Methods: Botanical samples of seven Aquilaria species were collected, and endophytic bacteria were isolated from surface-sterilized-tissue samples. The 16S rRNA gene fragments were amplified using PCR method, and endophytic bacterial isolates (EBIs) were identified based on 16S rRNA gene sequence similarity based method. Results: Culturable, 77 EBIs were analyzed, and results of 16S rRNA gene sequences analysis suggest that 18 different types of endophytic bacteria are associated with (seven) Aquilaria species. From 77 EBIs, majority (36.4%) of the isolates were of Bacillus pumilusConclusion: These findings indicate that agarwood-producing Aquilaria species are harboring 18 different types of culturable endophytic bacteria.

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Eighteen (18) types of bacterial endophytes found in agarwood-producing Aquilaria species and total number of bacterial endophytes

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