Background: The purpose of present investigation to isolate β-sitosterol diglucosyl caprate from the rhizomes of Alpinia galanga. Methods: The methanolic extract of the rhizomes of plant Alpinia galanga was subjected to column chromatography and was eluted with ethyl acetate-methanol (99:1) to yield compound (AG5) β-sitosterol diglucosyl caprate. Various spectral techniques such as Ultraviolet (UV), Infrared (IR), Hydrogen Nuclear Magnetic Resonance (1HNMR), Carbon Nuclear Magnetic Resonance (13CNMR), and MASS spectrometry (MS), were employed to determine and elucidate. Results: Chemical and spectral investigation of extract furnished a new steroidal glycoside. Conclusion: The isolated compound β-sitosterol diglucosyl caprate could serve as a lead compound in synthesis of steroids.